FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_ju2572_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_ju2572_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5021600
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT18450.015.3403191
TAATACT15900.014.9716594
GTATAGG6350.014.1324211
ATAATAC18700.013.8368633
GTATTAA17000.013.6032541
TAATACC14500.013.3241034
TATATAC17600.013.1334883
GACTAAG8550.012.91186143
TATACAG14750.012.8643735
TATTATA22900.012.8067912
GTATTAG11050.012.8067391
GCATAGG28300.012.80006550
GTATAAT21700.012.7246891
CCCTGTG14700.012.6745722
GTATATA16550.012.5132521
TATACTG15650.012.34501655
GTATAAA25200.012.3270431
TTATACT23600.012.2796294
CCCCTAG38350.012.2402551
CTAATAC11000.012.2317873
Produced by FastQC (version 0.11.2)