FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_ju2505_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_ju2505_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5590691
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA18950.018.577571
TTATACT28950.015.8494644
CTATACT11000.015.6815444
GTATTAT19800.015.1653371
GTATTAC12800.015.0999681
TATACTG19900.014.90928655
TTAATAC12350.014.5260623
TATTATA23950.014.1174212
TTTATAC23450.013.8291833
GTATATG14000.013.8056841
GTATTAG11950.013.5746291
TCTATAC13000.013.5343783
TATACTT21700.013.5135885
GTCTTAT15150.013.4411791
TATACTA9700.013.1595645
ATTATAC28000.013.0604863
TAATACT16100.012.8569174
GTCTTAG7650.012.6326531
ACGTAGA64150.012.53617569
GACGTAG72850.012.50777669