FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_ju1975_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_ju1975_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12193356
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG45300.015.46021655
GTATATA47350.014.7966981
GTATTAA28200.014.441811
GTATAAT51100.013.5757511
GTATTAT46600.013.4795131
TTATACT56300.013.1749144
TAATACT40300.013.0979744
GTATTAC30250.013.0067331
TACACAG37650.012.6453785
TATACCG20250.012.4369985
TATACAG41400.012.4166285
GTATAAG26450.012.3961451
TACACTG39650.012.3555745
AGACGTA145200.012.336328569
GACGTAG116050.012.22311369
GTATAGG24900.012.0589271
CTATACT25550.012.0175744
CCTATAC20200.011.9553093
GTATTAG27750.011.9397971
TCTATAC35850.011.9329193
Produced by FastQC (version 0.11.2)