FastQCFastQC Report
Fri 22 Oct 2021
H553JAFX3_n01_pSC92_MtLeaf-B_u.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH553JAFX3_n01_pSC92_MtLeaf-B_u.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7424923
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC134650.18134868199979987TruSeq Adapter, Index 27 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT75890.10220981416238256TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)