FastQCFastQC Report
Fri 22 Oct 2021
H553JAFX3_n01_AtSPL16_ampCol-B_u2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH553JAFX3_n01_AtSPL16_ampCol-B_u2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19909703
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATGCC3764921.8909975703806332TruSeq Adapter, Index 1 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT251120.12612945557249147TruSeq Adapter, Index 1 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)