FastQCFastQC Report
Sat 14 May 2022
H53WNDRX2_n01_Parse_Mini_Zm_Fixation_trials_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53WNDRX2_n01_Parse_Mini_Zm_Fixation_trials_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489920146
Sequences flagged as poor quality0
Sequence length74
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCGACCCCAGGTCAGTCGGGACTACCCGCTGAGTTTAAGCATAT39754860.8114559142868969Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGG33422820.6822095452265806Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGAGCAGCCCAGCTTGAGAATCGGGCGGCCTCGCCGCCCGAATTGT27896980.5694189191395285Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGTCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGA18991390.38764256083480186Clontech Universal Primer Mix Long (100% over 23bp)
GCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAA18279550.3731128460269523No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCC17664150.3605516152830343Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGG17446790.3561149738880099Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGAGCAGCCCAGCTTGAGAATCGGGCGGCCTCGCCGCCCGAATTG16203610.33073981815803916Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGAT15422580.3147978323798099Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGAGCAGCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAA14761610.30130645005155593Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAG14106840.28794161895926607Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTAC12196410.24894689674590356Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACA11248360.22959578396271949Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGT9641780.19680309288608025Clontech Universal Primer Mix Long (100% over 23bp)
CCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGC9324990.19033693707300617No Hit
GGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAG9314420.19012118762717709No Hit
CCAAGCTGGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTGAAATGCATTGAGATCGGAAAGAACAC9033700.18439127424655855No Hit
GGCGTCCTCAGCGACGGACCGGGCCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCC8935470.18238625361611482No Hit
GGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGG8869730.18104440228510219No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAAC8459490.17267079276221475Clontech Universal Primer Mix Long (100% over 23bp)
GTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCA8051100.16433494449521985No Hit
GCAGTGGTATCAACGCAGAGTGAATGGGGCGACCCCAGGTCAGTCGGGACTACCCGCTGAGTTTAAGCATATAA8018760.16367483691923948Clontech Universal Primer Mix Long (100% over 21bp)
GTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGATAGCGAAC7496480.1530143240935432No Hit
GGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGC7326660.14954804491750784No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACT7324310.14950007791677952Clontech Universal Primer Mix Long (100% over 23bp)
GGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGG6909930.14104196482665157No Hit
GCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGT6846620.139749713415541No Hit
GCAGTGGTATCAACGCAGAGTGAATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGGCT6806650.13893386617336612Clontech Universal Primer Mix Long (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACG6749720.13777184006636053Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGTAAAGCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACCAAAT6619870.1351214081325Clontech Universal Primer Mix Long (100% over 23bp)
GCCTCGCCGCCCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCTCTGGAAAGGGAC6535440.13339806605952473No Hit
CTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACG6396520.1305625019143426No Hit
GTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCC5997120.12241015293949556No Hit
GCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAG5898230.12039166072586859No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGAGGGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTCTGCCCG5858080.11957213941555284Clontech Universal Primer Mix Long (100% over 23bp)
GGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAATAC5781870.11801657978767831No Hit
CTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAA5735990.11708010064154414No Hit
GGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGT5704830.1164440786233763No Hit
GTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGG5687370.11608769401370975No Hit
GACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTT5566910.1136289259678658No Hit
CAGCGACGGACCGGGCCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCT5538700.11305311784422925No Hit
GGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGG5406670.11035818886288461No Hit
GTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAATACAGGCGAGAGACCGA5391360.11004568895601204No Hit
GTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGT5353150.10926576593565925No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGGGGCCTTCCCCGGGCGTCGAACAACCGACTCAGAACTGGTAC5347790.10915636035101932Clontech Universal Primer Mix Long (100% over 23bp)
CCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCT5330750.10880854856701483No Hit
CCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCC5282090.10781532547959358No Hit
GCCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGG5211200.10636835497677206No Hit
CCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAA5079430.10367873298274205No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAATA5038630.10284594420413974Clontech Universal Primer Mix Long (100% over 23bp)
GGCCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAG5025710.10258222775758234No Hit
GGGCGGCCTCGCCGCCCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCTCTGGAAA4990580.10186517212541818No Hit
GGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTC4965810.10135957952625203No Hit
CACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTA4951670.10107096106229524No Hit
GAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGC4900850.10003364915718325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)