FastQCFastQC Report
Sat 14 May 2022
H53WNDRX2_n01_Parse_Mini_Zm_Fixation_trial_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53WNDRX2_n01_Parse_Mini_Zm_Fixation_trial_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464664830
Sequences flagged as poor quality0
Sequence length74
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCGACCCCAGGTCAGTCGGGACTACCCGCTGAGTTTAAGCATAT47309111.0181340817207964Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCACGTGGAATCCCGTGTGAATCAGCAAGGACCACCTTGCAAGG42037430.9046828441911561Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGAGCAGCCCAGCTTGAGAATCGGGCGGCCTCGCCGCCCGAATTGT41668420.8967414211228338Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGTCAAAAGAGGAAAGGCTTGCGGTGGATACCTAGGCACCCAGAGA38875110.8366269080446652Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGAGCAGCCTAAACCGTGAAAACGGGGTTGTGGGAGAGCAATACAA31817640.6847438830263956Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGATAACATCATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGAT31392890.6756028856326398Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCGTAAAGCGTCTGTAGGTGGCTTTTCAAGTCCGCCGTCAAATCC27336250.5883003884757105Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGATTAGAGACCCCAGTAGTCCTAGCCGTAAACGATGGATACTAGG23742130.5109517326714828Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAG23494410.505620578170291Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGAGCAGCCCAGCTTGAGAATCGGGCGGCCTCGCCGCCCGAATTG22872500.49223652239830595Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGATAGATCATTGCAATTGTTGGTCTTCAACGAGGAATGCCTAGT18672350.4018455625315994Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCGAAAGCCTGACGGAGCAATGCCGCGTGGAGGTGGAAGGCCTAC18098800.38950225692785917Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACA16293430.3506490904422442Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGAAGTGGTGTTTCCAGTGGCGAACGGGTGAGTAACGCGTAAGAAC13313760.2865239445817322Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGTGAGTAACGCGTAAGAACCTGCCCTTGGGAGGGGAACAACAACT13039190.2806149542241017Clontech Universal Primer Mix Long (100% over 23bp)
GCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAA12098780.26037649546233144No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGCCGGGTCGTGCCTCCGGCGCCGTTACTTTGAAGAAATTAGAG10618570.22852106108396456Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGAGGGGCGCATTTATTAGATAAAAGGCTGACGCGGGCTCTGCCCG10467250.2252645202349401Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCAGAAGGGAGCTTGACTGCAAGACTCACCCGTCGAGCAGAGACG8657200.18631063599110784Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGGGGCCTTCCCCGGGCGTCGAACAACCGACTCAGAACTGGTAC8630650.1857392564012215Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGTCTCATGGAGAGTTCGATCCTGGCTCAGGATGAACGCTGGCGGC8335660.17939080950025849Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGGTAAAGCACTGTTTCGGTGCGGGCTGCGCGAGCGGTACCAAAT8184060.17612824280245182Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCGGCACATCTGTTAAAAGATAACGCAGGTGTCCTAAGATGAGC7627120.16414239915682882Clontech Universal Primer Mix Long (100% over 23bp)
GGCGTCCTCAGCGACGGACCGGGCCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCC7484110.1610646968913055No Hit
GGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAG7076900.15230117588197928No Hit
CCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGC7019320.15106200312169096No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCCCCTCCTTTTGGCTCCAAGGCCCGGCCTCGCCGGGCCGATCC6879060.14804348329956454Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGGAGCGTTCCGCCTTAGAGGGAAGCAAACGCGAAAGCGGGGGTCG6699410.1441772556791096Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGCGAGGCCGCCCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGG6606340.14217430658567382Clontech Universal Primer Mix Long (100% over 23bp)
GCCTCGCCGCCCGAATTGTAGTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCTCTGGAAAGGGAC6059540.1304066847495215No Hit
GGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCAGCCCAAATCGGGCGG5703360.12274137468075645No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCCGGGTCGTGCCTCCGGCGCCGTTACTTTGAAGAAATTAGAGT5403920.11629715982593304Clontech Universal Primer Mix Long (100% over 23bp)
GTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGCA5307870.11423007848474351No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCTCGAAGCGATACTGA5268940.11339227029512865Clontech Universal Primer Mix Long (100% over 23bp)
CTGGAAAGGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACG5179410.11146550514701103No Hit
GGGACGCCTGGGAGGGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGG5084430.10942145115652503No Hit
GGTGAGAGCCCCGTCCGGCCCGGACCCTGTCGCACCACGAGGCGCCGTCAACGAGTCGGGTTGTTTGGGAATGC5080740.10934203907793064No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCATA4868900.10478305405640449Clontech Universal Primer Mix Long (100% over 23bp)
GCAGTGGTATCAACGCAGAGTGAATGGGGCGACCCCAGGTCAGTCGGGACTACCCGCTGAGTTTAAGCATATAA4833300.10401691042552114Clontech Universal Primer Mix Long (100% over 21bp)
GTCTGGAGAGGCGTCCTCAGCGACGGACCGGGCCCAAGTTCTCTGGAAAGGGACGCCTGGGAGGGTGAGAGCCC4782650.10292687742259297No Hit
AAGCAGTGGTATCAACGCAGAGTGAATGGGAATGCAGCCCAAATCGGGCGGTAAACTCCGTCCAAGGCTAAATA4730490.1018043478780178Clontech Universal Primer Mix Long (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)