FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19778639
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9254404.678987264998366No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1167120.5900911584462409Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG228750.059.9114918
AGAGCAC320900.056.71263515
AGCACAC248150.055.86218317
CACACGT163050.055.8133819
GAGCACA320600.053.52936616
CACGTAG104550.051.2033421
ACACGAG92000.048.71781520
CACACGA93500.047.93540219
ACACGTC83100.046.6935620
TAACTGA211750.046.5333142
AAGAGCA479800.045.60490814
GTGACTG104950.045.3759771
ACACGTA114600.045.1554420
CGTGTGC102050.043.5762917
ATAACTG229250.043.134511
ACGTGTG104950.043.10550316
TGACTGG113400.042.0718652
ACTGAAG233900.040.986594
CACGAGA111400.040.4854221
CAGACGT117750.039.8860213
Produced by FastQC (version 0.11.5)