FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_330-1037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_330-1037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9727537
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1510501.5528082802460685No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1018111.0466267051978317Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG109200.054.7383818
AGAGCAC171650.052.35684615
GAGCACA170950.051.75289516
AGCACAC125950.051.57111417
CACACGA64800.050.982819
ACACGAG65900.049.8662720
GATCGAG49800.048.0672158
AGATAGA184000.046.351097
GATAGAT182750.046.3425838
CACGAGA71550.046.1241921
CACGTAG52100.044.9381421
CACACGT59900.044.7536719
AAGAGCA276300.042.81126414
ATAGATC204700.041.5953039
ACACGTA55650.041.50548620
CAGATAG71650.041.1756366
AGATCGA66900.037.2980087
GCAGATC110350.037.106815
ACGAGAT89600.036.8324322
ATCGAGA65800.036.6448869
Produced by FastQC (version 0.11.5)