FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_330-1034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_330-1034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8072016
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1380401.7101056291266021Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1181321.4634757909300478No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG120900.057.34452418
CACACGA68050.055.7487219
AGAGCAC189950.055.34566515
AGCACAC136250.054.83970617
AGATAGA223500.054.7881627
GAGCACA188250.054.6384516
ACACGAG70000.054.34570720
GATCGAG60500.053.9114278
GATAGAT228850.053.4308788
CACGTAG53100.051.4742821
CAGATAG87850.050.8316966
CACGAGA75150.050.5282821
CACACGT63900.049.01804419
ACACGTA55900.048.64553520
ATAGATC255500.048.1453939
AAGAGCA291650.048.00976614
AGATCGA71950.047.083087
ATCGAGA71400.045.8282979
TAGATAG76850.045.174516
TCGAGAT77350.042.3935310
Produced by FastQC (version 0.11.5)