FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_329-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_329-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10425351
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1374641.3185551258657862No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1333901.2794773048888235Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG120900.056.7655318
AGAGCAC215850.056.34142715
GAGCACA214450.055.682216
AGCACAC138800.054.03387517
ACACGAG73200.052.4958120
CACACGA73600.052.4007119
AAGAGCA359000.049.1316314
CACGAGA79800.048.1979121
AGATAGA195700.047.7467087
GATAGAT199000.046.902178
CACGTAG51900.046.86524221
CACACGT66050.045.67387419
ACACGTA54550.044.33193620
GATCGAG51500.044.238138
AGAGAGA216400.043.5192615
GCACAAG94700.043.46010618
GAGAGAT216500.043.0312116
GCAGATC140450.042.2861065
ATAGATC222550.042.0648359
CAGATAG80100.041.2443586
Produced by FastQC (version 0.11.5)