FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_329-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_329-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11104094
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1311121.1807536931873954No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC738360.6649439386950434Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG73700.053.18443318
AGAGCAC135600.050.86852615
GAGCACA134050.050.64876616
AGCACAC89850.047.24726517
ACACGAG50300.045.85127320
CACACGA50850.045.4929919
AAGAGCA249650.040.89093814
CACGAGA57150.040.35553721
CACACGT42700.038.1117619
AGATAGA128850.037.2373857
GAGAGAT161900.037.09406316
GATAGAT129600.036.724528
AGAGAGA165650.036.52799215
GCACAAG66250.036.2914918
CACGTAG38100.035.8239621
AGAGATC169000.035.22504417
ATAGATC138050.034.2736669
GCAGATC103400.034.117865
CAGATCG224900.033.378286
GAGCAAG92000.033.09522616
Produced by FastQC (version 0.11.5)