FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_329-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_329-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG841.1128775834658187No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC750.9936406995230525Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG150.00213948170.020
CACACGA150.00213948170.019
CACGAGA150.00213948170.021
ACGAGAT150.00213948170.022
AGCACAC207.4271156E-570.017
CGAGATC150.00213948170.023
GCACACG207.4271156E-570.018
AGATATC150.00213948170.07
GATATCA252.2406704E-456.0000048
AAGAGCA252.2406704E-456.00000414
GAGCACA252.2406704E-456.00000416
AGAGCAC252.2406704E-456.00000415
AAGATAT200.00668711752.56
TATCAAA200.00668711752.510
ATCGGAA955.7298166E-933.1578989
TCGGAAG1106.384653E-1031.8181810
CGGAAGA1106.384653E-1031.8181811
GATCGGA952.247034E-729.4736868
AGATCGG1003.5225094E-728.0000027
TGTAGGG657.242571E-426.92307718
Produced by FastQC (version 0.11.5)