FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_329-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_329-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7054314
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG968601.3730605130420903No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC188400.2670706180643504Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17050.038.794418
GAGCACA37800.032.40468616
AGAGCAC39550.031.76544215
GGGGGAT23400.029.5145971
ACACGAG14850.026.15942220
AGCACAC27850.026.13784217
CAGATCG61250.024.7397276
ATAGATC55450.024.6762459
CACACGA15900.024.43191119
GATAGAT56250.024.3876828
AGATAGA60100.022.9418667
AGGGGTA22550.022.8537371
GGGGGAC15400.022.76491
GGGGTCA16400.022.6518422
GGGGTAA25700.022.6368772
AGGGGTT26850.022.4579751
AAGAGCA91350.021.91262814
GGGGGTA23700.021.3010311
GGGGTTA22550.021.1365412
AGGGGTC13700.020.4717941
Produced by FastQC (version 0.11.5)