FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_329-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_329-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10978288
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3124262.84585356113813Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1471051.3399630252002863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC339350.057.97770315
GAGCACA337600.057.51218816
AGATAGA370000.057.4974257
GCACACG197150.056.87502718
AGCACAC219450.055.81595617
GATAGAT382400.055.4682438
CACACGA113150.055.2708219
ACACGAG114050.054.8653520
AAGAGCA535400.053.9711814
CAGATAG145750.053.328336
GATCGAG99450.051.5174458
CACGAGA122350.051.1719921
AGAGAGA334400.050.14972315
ATAGATC424000.050.0343259
GCACAAG134900.049.4725918
CACACGT100250.049.36179419
CACGTAG82800.049.11359421
TAGATAG118650.048.814436
ACACGTA84050.048.34153420
GCAGATC229500.048.1415185
Produced by FastQC (version 0.11.5)