FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_329-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_329-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12386826
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1871171.5106129689720353Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1716951.3861097265756377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG119000.055.2010718
GAGCACA214850.054.60077316
AGAGCAC217000.054.45969415
AGCACAC136850.052.041517
ACACGAG76100.051.4606920
CACACGA77900.050.09190819
AAGAGCA367450.048.41848414
AGATAGA237150.048.2091947
CACGAGA82950.047.29545221
GATAGAT243400.046.8562668
GATCGAG63200.045.5154238
CACACGT61300.044.0173719
AGAGAGA252200.042.80691515
GCACAAG100050.042.744918
ATAGATC269350.042.4847349
CACGTAG54850.042.30260521
CAGATAG96600.042.1317946
GAGCAAG130600.041.50863316
GAGAGAT259400.041.4458916
GCAGATC169600.040.2367745
Produced by FastQC (version 0.11.5)