FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_329-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_329-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9645985
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1311491.3596226823906528No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC460810.47772207814961354Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29450.041.4737218
AGAGCAC60150.040.6689715
GAGCACA61150.039.89047216
AGATAGA100300.034.7867287
AGCACAC40400.033.69776517
AGAGATC120600.033.40110817
GAGAGAT121300.033.35261516
GATAGAT106800.033.0626038
AGAGAGA127350.032.09707615
AAGAGCA127900.031.71279314
ATAGATC112900.030.8422649
GGGGGAT32650.030.2814141
ACACGAG25000.029.95757320
AAGAGAG145850.028.6976514
CAGATCG160200.028.3114326
GCAGATC78850.028.228975
CACACGA26700.028.18123819
GATCGAG27050.027.8155928
GGGGGAC27550.026.597141
AAGGGGG97200.026.50073667
Produced by FastQC (version 0.11.5)