FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_329-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_329-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11528598
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2536472.200154780312402No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC423430.3672866379762743Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG23400.034.54894318
GAGCACA48300.030.86747416
CGGGGAC14900.030.586581
AGAGCAC51550.030.07464415
CGGGGAG16600.027.2430321
AGCACAC35050.026.06099917
CGGGGAT28450.026.0000421
GGGGACC15400.025.0320722
AGATAGA89600.023.9036987
CGGGGTG17500.023.4380591
GATCGAG23900.023.428468
CGGGGTC17000.023.3025461
AAAGGGG96300.023.15054166
AAGGGGG96150.023.1138667
GATAGAT92350.023.0782078
ATAGATC93750.022.6589159
CGGGGTT23850.022.3423611
AGGGGGG106250.021.97075368
GGGGTTA33950.021.8837072
GGGGGAC24400.021.1203631
Produced by FastQC (version 0.11.5)