FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_308-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_308-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9503568
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1485621.5632234125120166Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1118441.176863258094223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG159750.058.120318
AGCACAC177200.055.9715817
GAGCACA215850.055.53157416
AGAGCAC220200.055.4028815
ACACGAG94100.055.19194820
CACACGA94850.054.7924319
CACGTAG67700.052.52142721
CACGAGA100150.051.9626721
CACACGT79950.051.1276519
ACACGTA69600.050.635120
GATCGAG56900.050.003268
AGATAGA223850.048.7772757
GATAGAT225400.048.240018
AAGAGCA308600.046.5521314
CAGATAG84700.045.6150326
ATAGATC255100.042.842949
ACGAGAT123200.042.1839422
AGATCGA71650.041.7120677
ACGTAGA86300.041.24217622
ATCGAGA72900.039.268439
Produced by FastQC (version 0.11.5)