FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_308-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_308-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9392445
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1204361.2822646286456827No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1018521.0844034753464087Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG106550.056.52795818
AGCACAC117450.054.02324717
GAGCACA144000.053.9057316
AGAGCAC147900.053.71331815
CACACGA60550.052.53945519
ACACGAG62400.050.86962520
CACGTAG49050.049.16047321
CACACGT58800.048.03215419
CACGAGA68000.046.5774321
GATCGAG43300.045.0185628
ACACGTA53800.044.42979420
AGATAGA172800.043.2190257
GATAGAT178150.041.7443248
AAGAGCA234050.041.61298414
AGATCGA52600.038.2566037
CAGATAG65800.038.1878436
ATAGATC199650.037.4943479
ACGTAGA66700.035.94186822
ACGAGAT88750.035.64806422
ATCGAGA57300.034.019269
Produced by FastQC (version 0.11.5)