FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_308-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_308-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10439410
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1312101.2568717963946239No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC844320.8087813391752982Illumina Single End PCR Primer 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG84600.055.9279918
AGCACAC94900.052.84486817
GAGCACA115850.050.81042516
CACACGA50300.050.78994819
AGAGCAC120050.050.75103815
ACACGAG53550.047.90351520
CACACGT46650.046.4367619
CACGTAG38750.046.42098221
CACGAGA58600.044.31279821
GATCGAG45400.043.8593988
AGATAGA172300.042.48967
ACACGTA41850.042.39702620
GATAGAT179700.040.6230438
CAGATAG62400.039.2577976
AAGAGCA202750.035.50436814
AGATCGA57900.035.4785427
ATAGATC215950.033.99849
ATCGAGA62500.032.0834359
ACGAGAT87550.030.01972222
TAGATAG70650.029.9678576
Produced by FastQC (version 0.11.5)