FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n02_308-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n02_308-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9892685
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2141712.1649430867353No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC594900.6013534242725812Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG46650.049.063318
AGCACAC54900.044.30402417
GAGCACA67950.042.3878216
AGAGCAC71700.041.4393215
CACACGT30300.039.3860519
ACACGAG30000.036.98015220
CACACGA30550.036.19983719
CACGTAG26750.035.58572421
GATCGAG23900.033.0928888
CACGAGA33550.033.067221
AGATAGA105000.032.6301967
GATAGAT110600.030.8829528
ACACGTA31950.029.79399520
CGGGGAC9650.029.4253861
CAGATAG45450.026.8732416
AAAGGGG116050.026.87081366
ATAGATC128300.026.6496819
AAGGGGG116850.026.53708867
CGGGGAT18250.025.5477831
AAGAGCA137600.025.4080414
Produced by FastQC (version 0.11.5)