FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19778639
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1228590.6211701421922914TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG891810.4508955343186152TruSeq Adapter, Index 15 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG399070.2017681803080586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT362650.044.515221
ACGTGTG550600.029.3210452
AAAAGGG213100.025.23518670
GTCACAG400450.023.59858130
AAAGGGG74650.022.5440270
GTGACTG54500.022.2815651
GTGTGCT730050.022.1856174
CGTGTGC732550.022.1194573
TCACAGA457850.022.00870531
CACAGAT505550.021.30254732
TCCGCTC104250.020.5391613
GTGCTCT808600.019.9698566
TGTGCTC817650.019.7873385
GGGTGGC70900.019.5911770
TGACTGG61500.019.1731222
ACAGATC565200.019.0157633
GCTTGAA340300.018.4834260
CCGCTCT120250.017.86462614
CTGCTTG341650.017.3955558
TGCTTGA355800.017.01866159
Produced by FastQC (version 0.11.5)