FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_330-1037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_330-1037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9727537
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG447640.4601781519823569TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG67900.041.3024641
AAAAGGG129550.031.41998170
GACGTGT27500.030.1737211
GTGTGCT142650.027.7022322
CGTGTGC151550.025.9671081
GGGTGGA67950.024.05420770
TGTGCTC176600.022.6343523
GCGTGGA59350.022.1143570
GTGCTCT183050.021.8750444
AAAGGGG33300.021.23101670
AAAAAGG213100.019.90594369
GTGGAAA103750.018.68896562
TGCGTGA61150.018.25819459
CGTGAAA78150.018.09322561
GTGCGTG41400.018.09160458
GCGTGAA76300.017.6603760
CGTGGAA61400.017.10080361
CTGCGTG70000.017.09982558
TGGGGGT90700.017.09464567
TGCGTGG44400.017.02685459
Produced by FastQC (version 0.11.5)