FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_330-1034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_330-1034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8072016
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG344580.4268822063781836TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATG180370.2234509941506558TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG74650.044.9299661
AAAAGGG141250.041.9499770
GACGTGT27050.030.933641
AAAGGGG30650.030.0322970
GGGTGGA92500.028.98345870
AAAAAGG215100.028.55616669
GTGTGCT157900.027.3324832
GTGGAAA112250.026.25360362
GCGTGGA76500.025.20886870
CGTGTGC171200.025.1945921
CGTGAAA79800.025.04357761
TGCGTGA65450.023.84999859
AAAAAAG296100.023.75859868
GCGTGAA83150.023.0664960
CGTGGAA70650.022.490961
TGTGCTC197150.022.015223
GGAAAAA174350.021.72043464
CTGCGTG79450.021.71781758
GTGCTCT203100.021.4047324
TGGAAAA161250.021.29278263
Produced by FastQC (version 0.11.5)