FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_329-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_329-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10425351
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG1479641.4192711593115666TruSeq Adapter, Index 22 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG80300.039.413751
ATGCCGG66450.032.55046570
GACGTGT35300.031.6381361
AAAAGGG173600.030.46340270
CGTGTGC159100.028.562711
GTGTGCT163100.027.813282
AAAGGGG59600.027.60039170
CGTCTTG79100.026.76964270
CTTGAAA428150.024.21323261
TGAAAAA497450.023.35889663
TTGAAAA467100.022.54633762
GAAAAAA537050.021.92976664
AAAAAGG254150.021.49693169
GCTTGAA479050.021.39212460
AAGGGGG32200.021.1954470
TGCTTGA490200.020.4628759
TGTGCTC227200.020.0938971
TCTTCCG523950.019.7276232
CTTCCGA513300.019.7139573
GTGCTCT233400.019.6157044
Produced by FastQC (version 0.11.5)