FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_329-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_329-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11104094
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG48200.034.2112431
CGTGTGC96400.027.2745691
GTGTGCT100600.026.060282
AAAAGGG116900.024.85006570
GTGTGCG27950.021.53825856
TCCGATC304250.020.8459115
GTGCGTG33800.020.70986458
CCGATCT304000.020.6673186
CTTCCGA315100.020.327973
TCTTCCG330700.019.6973322
TTCCGAT325800.019.5744954
TGTGCTC140050.018.8237821
GTGCTCT143250.018.4479262
CTCTTCC363900.018.0583131
GCGTGGA40750.017.3495360
GGCGGGG117850.017.13602868
CGATCTG126750.017.1203087
GGGTGGA58800.017.0236570
TGTGCGT45900.016.85169257
GCGTGAA42200.016.83633460
Produced by FastQC (version 0.11.5)