FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_329-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_329-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1491.9740328563857976TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320.4239533651298357No Hit
AGATCGGAAGAGCACACGTCTGAACTCCACCCACAGATCGGAAGAGCACA240.3179650238473768Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG190.25172231054584TruSeq Adapter, Index 22 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG180.23847376788553257TruSeq Adapter, Index 15 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACAGATCGGAAGAGCACAC140.18547959724430313Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCATTCACAGATCGGAAGAGCACA80.10598834128245893TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA150.00213948170.059
GCTTGAA200.00668711752.560
CTTGAAA200.00668711752.561
GAAAAAA351.8940937E-550.064
TGAAAAA351.8940937E-550.063
CCGTCTT305.5117975E-446.66666451
GCCGTCT305.5117975E-446.66666450
GTGTGCT503.0400333E-642.0000042
TTGAAAA350.001177707740.062
TGTGCTC458.357403E-538.888893
CGTGTGC601.0636737E-535.01
GTGCTCT552.7213152E-431.818184
TTCCGAT604.5333029E-429.16666610
GCTCTTC604.5333029E-429.1666666
TCCGATC604.5333029E-429.16666611
CCGATCT604.5333029E-429.16666612
CTTCCGA604.5333029E-429.1666669
TGCTCTT657.242571E-426.9230775
CTCTTCC750.001669527523.3333347
TCTTCCG750.001669527523.3333348
Produced by FastQC (version 0.11.5)