FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_329-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_329-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7054314
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG274690.3893929303402145TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG16500.018.2474371
CGTGTGC26600.017.5048071
TCCGATC67850.017.2300055
AAAAGGG66800.017.13308170
CTGCTTG84650.016.99335158
CCGATCT69150.016.6530026
TGCTTGA91900.016.30018259
GCCGTCT104050.016.07866550
GCTTGAA98850.015.54361460
GTGTGCT30450.015.4030352
CTTCCGA77100.015.1628513
CCGTCTT113650.014.90527851
TGCCGTC125500.014.8644149
ATGCCGT131150.014.67771848
CATCTCG145700.014.41304940
TTCCGAT81650.014.2750244
TATGCCG137550.014.27468247
GTATGCC146600.014.25294246
ACATCTC156650.013.76304339
TCTCGTA154300.013.70046142
Produced by FastQC (version 0.11.5)