FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_329-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_329-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10978288
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTG330140.30072084099087215TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG279090.2542199658088766TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG265750.24206870870940897TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG244910.2230857853246335TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG241960.2203986632524124TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATG205590.1872696362128594TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGTTT173170.1577386200835686TruSeq Adapter, Index 10 (97% over 42bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152140.13858262781956532No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATG132890.12104801768727509TruSeq Adapter, Index 10 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG221350.046.56610570
ACGTGTG122900.042.4146351
GACGTGT49650.036.9474951
GTGGAAA226500.030.28670362
CGTGGAA112950.029.8093861
CGTGTAA43500.029.12617561
GTGCGTG108950.028.07684758
GTGTGCG92150.027.80224656
GTGTGCT264400.027.7604712
GTCTGCG62800.027.64306456
TGCGTGA85650.027.58294159
CGTGAAA110250.027.33308661
TGTGCGT120900.026.92283457
AAAAAGG398850.026.78175769
GCGTGAA105750.026.74207760
TGCGTGG101950.026.02232759
CTGCGTG97650.025.87790558
CGTGTGC287200.025.5615371
GGGTGGA201650.025.49691870
TGGAAAA310200.025.31892263
Produced by FastQC (version 0.11.5)