FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_329-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_329-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12386826
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG2200701.7766456071959031TruSeq Adapter, Index 9 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG69350.040.0296021
AAAAGGG233250.033.56676570
CGTGTGC138900.031.6045671
ATGCCGG57400.031.34125770
GTGTGCT146900.029.783532
AAAGGGG77100.028.87140370
CGTCTTG84750.028.37149270
CTTGAAA608750.026.48195861
TGAAAAA687100.025.46409863
TTGAAAA650750.025.07935562
GAAAAAA738500.023.88135564
AAAAAGG344200.023.55010469
GCTTGAA692800.022.97617360
AAGGGGG36850.022.41504770
TGTGCTC216250.021.9511951
TGCTTGA716900.021.8669259
CTTCCGA548950.021.5000363
TCCGATC547150.021.3532685
GTGCTCT222550.021.3107952
TCTTCCG563250.021.2588522
Produced by FastQC (version 0.11.5)