FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_329-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_329-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9645985
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC172050.025.5519545
CCGATCT171950.025.0986336
CTTCCGA178150.024.8342133
TCTTCCG190400.023.6042122
ACGTGTG23700.023.487391
TTCCGAT189900.023.2054484
CTCTTCC214400.021.0155011
CGTGTGC46100.019.8969441
CGATCTG74450.019.60397
CGATCTA59650.019.3630897
AAAAGGG94450.019.12106770
GTGTGCT48550.018.9608552
TGTGCTC64100.018.4059331
GTGCTCT67150.017.5139732
GCGTGGG31900.017.22556160
CGATCTC52700.016.9356067
CGGGGGT60750.016.76529161
GGGTGGA47700.016.5826770
GTGTGCG22500.015.86653356
TGCGTGG26800.015.80210759
Produced by FastQC (version 0.11.5)