FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_329-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_329-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11528598
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC84550.016.1040065
AAAAGGG107300.015.98307970
GTGTGCG20050.015.88514156
GTGCGTG23050.015.79161358
GCGTGGA33550.015.54382260
ACGTGTG24200.015.3341631
CCGATCT86400.015.2730436
TGCGTGA34050.015.21278359
CTGCGTG36850.015.19660558
GCGTGAA39350.015.03162960
GTATGCC98350.014.76854846
GCCGTCT46850.014.64234250
TATGCCG88450.014.56176647
GTCTGCG23400.014.50841356
CTTCCGA98750.014.107243
TGCCGTC67200.013.80195649
TGCGTGG29300.013.73707559
GTCACAC295850.013.41545930
ATGCCGT75950.013.3178548
CGTATGC106700.013.2847945
Produced by FastQC (version 0.11.5)