FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_308-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_308-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9503568
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1587211.670120106469486TruSeq Adapter, Index 4 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG94650.046.199461
AAAAGGG160400.037.1817770
GACGTGT33200.034.693881
GTGTGCT200000.030.0486982
AAAGGGG47200.028.69682170
CGTGTGC214400.028.0539111
TGTGCTC230050.026.3060633
GTGCTCT235850.025.8372334
TGAAAAA452750.025.66518663
CTTGAAA376050.025.2876761
AAAAAGG243450.025.20211269
TTGAAAA405900.023.95399762
GAAAAAA499300.023.56681864
GCGTGAA91600.022.39066370
GCGTGGA38550.021.97130270
GCTTGAA432300.021.68154160
CGTGAAA82300.020.1578161
TGCGTGA77100.020.1554969
TGCTTGA450850.020.0442159
GCTCTTC311600.019.6572936
Produced by FastQC (version 0.11.5)