FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_308-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_308-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9392445
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG263370.28040622010562744TruSeq Adapter, Index 3 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG74300.043.4432261
GACGTGT28200.036.7470551
AAAAGGG123300.034.1765170
GTGTGCT146100.026.6409032
CGTGTGC149300.026.0515881
GGGTGGA68200.024.53058270
TGTGCTC167650.023.3207073
GTGCTCT170900.023.0205824
TGCGTGA48950.021.45027759
AAAGGGG29500.021.23710670
AAAAAGG209200.021.21396869
GCGTGGA52650.020.80704170
GCGTGAA64550.019.95335460
GTGCGTG39300.019.59270958
GTGTGCG33800.019.46728956
CTGCGTG53000.018.75455758
CGTGAAA71750.018.68276661
GTGGAAA104550.018.37860962
TGCGTGG38100.017.91323359
TGTGCGT53350.017.64745957
Produced by FastQC (version 0.11.5)