FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_308-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_308-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10439410
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG220070.21080693257569155TruSeq Adapter, Index 2 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGCGTATG111600.10690259315421081TruSeq Adapter, Index 2 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG58850.040.5712471
GACGTGT25600.034.735551
AAAAGGG116800.030.86443570
GGGTGGA70150.027.0916970
GTGTGCT119750.025.955692
CGTGTGC127950.024.187581
TGTGCTC130500.023.8712223
GTGCTCT136550.022.8904824
GCGTGGA55050.021.61650870
TGGGTGG50300.019.13500869
TGCGTGG44200.019.0835359
AAAAAGG203100.018.66304469
GTGGAAA103250.018.60999362
TGGGGGT77300.018.42802667
GGGGGGA23850.018.34364370
GTGTGCG37150.017.71181156
TGTGGGT63300.017.58277367
AAAGGGG29350.017.52964670
CTGCGTG62250.017.42955658
GCGTGAA64100.017.41793360
Produced by FastQC (version 0.11.5)