FastQCFastQC Report
Thu 29 Nov 2018
H53MJAFXY_n01_308-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53MJAFXY_n01_308-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9892685
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG666370.6735987247142712TruSeq Adapter, Index 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG34150.032.1903881
CGTGTGC55050.024.4208721
GTGTGCT58800.022.858222
AAAAGGG108550.022.69902270
AAAGGGG33700.020.04433870
TGTGCTC68700.019.5642383
GACGTGT19050.018.7453021
GCTTGAA244050.018.67224360
GTGCTCT75250.018.0938744
TGCTTGA243650.017.94156659
CTTGAAA269500.017.37647461
CTGCTTG251500.017.2284858
TGCCCCG21000.016.667455
GTGCCCC22300.015.8527644
TGAAAAA340700.015.75859763
CCCCGGG22300.015.2241627
AAAAAGG173550.014.88319169
CCCGGGT23100.014.6968438
TTGAAAA325950.014.63555462
GCGTGAA37100.014.62251670
Produced by FastQC (version 0.11.5)