FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38284075
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24666536.443026245246881No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN609070.15909225964059467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA58100.034.209386
CGGAGAT69900.032.1928639
TCGGAGA66500.031.8653418
CTCGGAG73350.026.6042757
TACACTG121450.015.2248385
GGCTCGG121250.013.9891315
TATACTG157750.013.9365135
GTCCTAG61050.013.0989631
CTAGACT74750.012.7553824
CTACACT111150.012.1797084
GTATTAG112250.012.1759081
CTAGCCT77550.011.7317164
TAGACTG95250.011.6934585
GGGCTCG114050.011.6785564
TCTAGAC81600.011.5922953
CTAGGAC59950.011.4091923
TAGACAG170200.011.3772845
GTTACAC69300.011.3398353
ACTGTGC110400.011.2131358
CTATACT116750.011.0967664
Produced by FastQC (version 0.11.5)