FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_Neg.2.21_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_Neg.2.21_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTAAAAGTTTGCTATTAAAGAGAAGGTTCCTAAAAGGAAGGGCATCAC11.4925373134328357No Hit
CAAAAAGAACGTGTTTTTAGATTCTGAGAGACCTAGTTATAAATTATTTA11.4925373134328357No Hit
CTCTTACGCTTCTAATCACTGGGAAGTTCAGGAAATTGGAAACGCCCCAG11.4925373134328357No Hit
CTCTGAGTCTATGTTCATTTCCTTCATTCTTTTTTTGATTTTCTTCAAAT11.4925373134328357No Hit
TTGTAATTGTGTAAATCATTAGATTCAGTTTGCATTGTAGCAACTTGAAG11.4925373134328357No Hit
ACACAACACGGTGGCTGGCACATAGTAGATGTTTAATAAATAATAAATAA11.4925373134328357No Hit
GCAGTAGAGAGAGAGGTGGAGGAAAACAATGTTAGACTACAGGGTCTTTC11.4925373134328357No Hit
TCTCACAGGTGGCCACTCTTAGAGGCAATACATGGCAAATGTTTTCTATT11.4925373134328357No Hit
GTCCAGCGTTCTGAGCTTCTGAATTTGGACAGTGGTGATTGAACATGGAC11.4925373134328357No Hit
CCCAGGCACCAGCCTAGAAATCTCTTGCCTTCCAAGCTGGAGTCTCCACC11.4925373134328357No Hit
GTCTACATGAGCAGTGAAGTTCAGCAACTGCTTTACAACAGCGTTTTCAA11.4925373134328357No Hit
CAGAATATCAAACATGACAAAAAAGACGGCAAAAAACCGAAAAAAAGTAT11.4925373134328357No Hit
ATGTGACACAGAGACACTGCGTGAGCTTAGGCTGTTGGCAAAATAATGTT11.4925373134328357No Hit
CTAAATTACATTACATTACACTACAAACCCATATAAACCATTCAAATCAA11.4925373134328357No Hit
ATGATAAAGTACTTATCAAACACTAAGAAGTGTGTATTTTTATTCATTAT11.4925373134328357No Hit
GTACATGGAAGTATCCACCACATGGACTAATAATAACAGGTGCTCAATAA11.4925373134328357No Hit
CCTGTAATCCCAGTTACTTGGGAGGCTGAGGCAGGAGAATAGATTCAATC11.4925373134328357No Hit
TTGTAGCTCAGTGTGAGTTCTTCAGGAGGGGAACATGGTCTGCAAATGTG11.4925373134328357No Hit
GATAACCAGCTCAAATTGTGAAAGTTTAAGGAATATCATGAAACATTTCC11.4925373134328357No Hit
AATTTACAATTGTCAAGTCTCAAAGAACTTATGCAGACGTAATACGTTGT11.4925373134328357No Hit
GCCCACTTGAAGGGGAAGAAACTAGGTATTTGAAAGGCACTAAATTATCC11.4925373134328357No Hit
GGGTAGAACATTCTGACACCAAGCTTAGGGGCACTCCTACTAATGAATTT11.4925373134328357No Hit
CCATTCAGCAAACATGTTGAGTGTTTACTGTATGTCTGTCATTTTCTTGG11.4925373134328357No Hit
ATGAATGTTGAACAAGCATTGAATCCCAAGTATTAAGCAGAATAAATCAT11.4925373134328357No Hit
GTATTGTAACTTTTAACATTCTATGTAGTCTTTGCCGCCATCGGCGTGCC11.4925373134328357No Hit
CTTATACACCTCTGACGCTGCCGACGATATGCTGTGTGGGGGGCTCGGTG11.4925373134328357No Hit
GTCATAACAAAATACCACTGACTGGGTGGCTTAAACACCAGAAATTTATT11.4925373134328357No Hit
CCTTTTGACAGAGCAGTTTTGAAGCACTCTTTTTGTAGAATCTGCAAGTG11.4925373134328357No Hit
GGTCAGGAGTTCCAGACCAGCCTGGCCAACATGGTGAAACCTTGTCTCTA11.4925373134328357No Hit
AGCGAGCTCTCACCACAACCTGACCATGATGGGACCTGGTAACAGCCTTC11.4925373134328357No Hit
GACCAACATGGTGAAACCCCATCTCTCCTAAAAATACAAAAAATTAGCCA11.4925373134328357No Hit
GATTACATTGTGAGTCTAAACAAAATGTAATATTATAGCAAAAGAACAAA11.4925373134328357No Hit
TTCCCGGTGCGACCGTCACCCCGTTCTTTGACTATGAAAGGGCACGCCCT11.4925373134328357No Hit
TTATAGGGGTGGGGCAAGGTTGAGATCTAAACACGGGGCTTGGGCTCCCC11.4925373134328357No Hit
CATGAAGATGCTGTGGCAGCTATGTCAAAAGACAAAGCAAATATGCGTAA11.4925373134328357No Hit
AGGAAAATCACTTCAACTTCTGGGTCTGTTTCTTTATCCATAAAATTAAA11.4925373134328357No Hit
GGCCCAGAAAAACATTCTGAACCAGCGAGGCTCACACCCCATTTCCCATG11.4925373134328357No Hit
ACAAAAAACAATTTCTAATTTACATAGTTTATAATGTGATATATAAAAAA11.4925373134328357No Hit
GACTTTAAATTCTCTTATGGGTGAATTTTAAAAATGTATATCAGTTGCTT11.4925373134328357No Hit
AAAACAAACTTTTCAATAATTAGAATAATTATGAAATAACTAAAATATCA11.4925373134328357No Hit
AGGCAGGCTAGTCACAAGGGAAGAAGGGGTTTGTTTTGCTAAAGGGCCAT11.4925373134328357No Hit
ATCTTGTAATTTTAAGGTTAAACAAGGATGTGTGATGTGTGCACATCCAT11.4925373134328357No Hit
GAGAGAGAGAGAAGGGTTGAAAATATAAGCATTGAAAATGTTCTTTCCAT11.4925373134328357No Hit
GAACAAGGGAGGGTAACTAGAAACACAGCAGCAATCTCAATCATTATAAA11.4925373134328357No Hit
TTAGCAAAATCATAAAAGAAATATGTCTTCTCCAAAGTGTGCATATAATT11.4925373134328357No Hit
GACTCACACTCCCGAGTAGCTGGGATCGCAGGCGTGTGCCACAATGCCCG11.4925373134328357No Hit
CACATGGGATACAGTCCTGGTAATTTCTATACTGGAGGATTCTAAGATGA11.4925373134328357No Hit
TAAATAAATAAGATATAATCATGATATGGTGTTATCTGTTTCTAAGGGAA11.4925373134328357No Hit
CACATTAACTTTCCTATTATAGTGATTCTATATTAAAAGTGGATCAAAAA11.4925373134328357No Hit
ACCCAATAAAGCTCAAGTTGTTCTCATTCATTAACTACATTCAGGAAAGA11.4925373134328357No Hit
CAAAAAGGCGTAGGAAAAGAATTAAGAATAATTCAGGCAAAATGCCTCCT11.4925373134328357No Hit
CTCTGAATGGGCTGGGACCTGTGGAGAGGGACCAGTGAGGGCTCAAGGGA11.4925373134328357No Hit
CATAAAAACTCTCCAGATAAACTTTGGCACTCAGAACAATCAAACCATTA11.4925373134328357No Hit
TTCCATTCCATTCCCCTGCAGTCGGGTTTATTCCATTCCATTCCATTCCA11.4925373134328357No Hit
GCACGGTGGCTCAAACCTTTAATCACAGCACTTTGGGAGGCGCAGGCGAG11.4925373134328357No Hit
TAGTAGAGTTGATACGTAACATATAGAGTGAGACGAGAGGATAGCATAAC11.4925373134328357No Hit
CAAATCACTACATGTATAAATAGGATACAATAAAAAACAATTTGAACATT11.4925373134328357No Hit
CTATAATTGTAAGCAAAAAATAATAAATACATAATCTAAATCATAAAAAA11.4925373134328357No Hit
CTCAAGAAAATAAAAACACCATCACACAATATTTAAAAACAAAAAATATA11.4925373134328357No Hit
GACTGACCGAGGGCAGTAGGCTCACCTGGGACCAGGGCCTGTTAGCAAAG11.4925373134328357No Hit
TAATAAAATTGAGATGTTCTTCTCCCTTTCTCTCACAAATGAATTCTAGA11.4925373134328357No Hit
CTCCTGAAGCTCAGATTTATTTAAAATGTGTCTCTGAAGGACAGTAGGTG11.4925373134328357No Hit
GATAAAACATTATTTCTTTTGCAGCATTCAGAGAACTGAGACTCTCTCTT11.4925373134328357No Hit
GTGTTCAACAGATCGCATAAACATGCCAAAAAAATACTTGCGTGATGAAA11.4925373134328357No Hit
TCCTACTTCCTACCTCTGGGGCTACATAGCTTGAATCCTCATTAAATAGA11.4925373134328357No Hit
CGCCCGGCTAACGTTTTATATTTTGAGTAGAGACGGGTTTATGTCTCTTA11.4925373134328357No Hit
GTGGAGCTCTTCCCAAGAGGCGATGAGTCGACTCACTGAGTCGAAGAGCA11.4925373134328357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGT50.0145.0145
AGCTCAG50.0145.08
CTCCTGA50.0145.01
GAAGCTC50.0145.06
CCTGAAG50.0145.03
CTGAAGC50.0145.04
TGAAGCT50.0145.05
TCCTGAA50.0145.02
AAGCTCA50.0145.07
GCTCAGA50.0145.09
Produced by FastQC (version 0.11.5)