FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_MG_30908.TR4_batch_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_MG_30908.TR4_batch_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11341662
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128640.11342253013711748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA33850.064.269266
TCGGAGA37250.058.9928328
CGGAGAT39850.055.3280539
CTCGGAG44750.046.187167
GGCTCGG39900.044.7082445
GGGCTCG34200.041.9773144
CGTGGGC41250.024.600051
TACACTG98400.015.6230625
TATACTG138250.015.4208255
GTGTAGG57700.014.9486881
TACACCG16300.014.6808685
GTCTTAG59250.014.6799581
CTATACT87050.014.659514
GCTACAC45050.014.6441683
CTACACT70200.014.5632264
GTATAGA83450.014.5051441
GTGTTAG82600.014.2156541
GTATAGG48450.013.7634071
TAGACTG75650.013.6114635
TAGACAG146200.013.4414865
Produced by FastQC (version 0.11.5)