FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_9424.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_9424.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14193000
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG111800.016.9934045
TATACTG151600.015.9281735
CTATACT103650.014.8299624
GTATAGA106700.014.6732071
GTATAGG59300.014.3010161
TATACCG28400.014.0431745
GTGTAAG92050.014.01625251
CTACACT91500.013.708774
CTTACAC88350.013.6217743
TACACCG38500.013.5610295
GTACTAG45350.013.1060641
TAGGACT80150.013.0266414
GTACAGG85700.013.0249371
GTATTAG109950.012.9210121
TCTATAC103100.012.86843
GTGCTAG68350.012.7256041
GTGTAGG74300.012.6820731
TTACACT131650.012.6671944
TACACAG142300.012.6376915
GTCTTAG75600.012.3681181
Produced by FastQC (version 0.11.5)