FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_8336.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_8336.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15060836
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA46600.053.9960756
TCGGAGA56650.046.9813238
CGGAGAT58150.045.896429
CTCGGAG56750.045.108297
GGCTCGG51600.043.1406945
GGGCTCG46250.039.1909984
CGTGGGC54750.019.9913771
TATACTG164700.015.1007665
TACACTG125500.014.44424255
TACACAG151150.014.0079055
GTGTAGG76700.013.8922261
CTACACT99950.013.8550674
TACACCG33250.013.5206795
CCTACAC74600.013.4113023
TGGGCTC147300.013.3382013
GTATAGA127200.013.2205881
GTGTTAG109050.013.16101551
TATACCG26300.012.9580775
CTATACT118150.012.9481224
GTATTAG123400.012.8640831
Produced by FastQC (version 0.11.5)