FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_8230.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_8230.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15125718
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167210.11054681833946661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA64900.088.7149356
TCGGAGA69700.086.468258
CGGAGAT73650.084.791139
CTCGGAG75300.075.699257
GGCTCGG69050.072.982315
GGGCTCG59100.068.086624
CGTGGGC57800.044.3926471
GATCGGA14650.023.75886
TGGGCTC188300.022.0992553
GTGGGCT127450.021.5560632
TGGCTCG34550.020.3554134
TGCTCGG34600.017.8130935
ATCGGAG21300.017.7046667
CGGCTCG19950.017.080924
GTGTAGG74950.016.3437121
TCGGATA17850.016.2521278
TATACTG193700.015.9094735
TACACTG136300.015.9063985
GCTCCGA21900.015.8934456
CTACACT101350.015.8097414
Produced by FastQC (version 0.11.5)