FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_8230.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_8230.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14853912
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151630.10208085250538712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA58450.088.953236
CGGAGAT65500.083.708689
TCGGAGA65300.083.1845258
CTCGGAG72650.071.372067
GGCTCGG63800.071.146885
GGGCTCG55200.067.117054
CGTGGGC57850.043.227051
GATCGGA14950.021.342176
TGGGCTC183200.021.2500693
GTGGGCT125800.020.6293092
TGGCTCG33450.019.2905644
TATACTG177250.016.7725775
CCGACGC111150.015.712652145
TACACTG134300.015.6575655
GCCGACC31400.015.462761145
TGCTCGG33450.015.3909155
ATCGGAG20900.014.9203837
GACGACA36450.014.11571145
GCACGGA26500.013.9556926
CCTACAC73950.013.7246543
Produced by FastQC (version 0.11.5)