FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_8230.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_8230.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034186
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198470.11005209772151624No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA85450.074.7624056
CTCGGAG95100.067.029567
CGGAGAT102850.065.863929
GGCTCGG84450.064.568875
GGGCTCG69000.063.675794
TCGGAGA110400.059.71418
CGTGGGC80600.037.3193171
TGGGCTC174650.025.5278323
GTGGGCT129250.023.6669522
TATACTG180950.015.6683455
CCTACAC89350.015.4969483
TACACCG56100.015.1226535
TATACCG42500.015.0140815
GTATAGG80850.014.8815711
GATCGGA24150.014.7129046
TACACTG130250.014.5300715
CTACACT112800.014.4618624
GTATAGA140900.013.7347561
GTATTAG159700.013.6609921
CTATACT133000.013.5191944
Produced by FastQC (version 0.11.5)