FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_7920.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_7920.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13875352
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA22950.031.2804186
TCGGAGA27500.026.8986118
CGGAGAT31500.023.944669
CTCGGAG33050.022.6003727
GGCTCGG31750.020.097565
GGGCTCG28450.018.8588284
TATACTG176850.017.7126315
GTATAGA105800.016.3056871
CTATACT109200.016.1342534
TACACTG122500.015.9820365
CTACACT90950.015.06691554
TACACAG162350.014.7389335
CCTATAC56050.014.3575823
ATACTGT220950.014.3419276
TCTATAC108050.014.2247623
ACTGATC91800.014.2197578
CCTACAC68700.014.1410613
GTGTAGG69300.013.8066561
TAGACTG95400.013.7573455
TCTGTCG48550.013.7423488
Produced by FastQC (version 0.11.5)