FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_7651.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_7651.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14093173
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA30050.050.434926
TCGGAGA32750.047.167478
CGGAGAT34550.044.5025759
CTCGGAG38950.038.5406157
GGCTCGG36950.035.520945
GGGCTCG32700.034.8120044
CTACGAC8950.017.821193
TATACTG186650.016.938655
TACACTG129500.016.7985485
CGTGGGC40100.016.6293661
CGACGAA10550.016.485188145
GTATAGA110150.015.5953841
TACGACT11950.014.5619814
ATACTGT225400.014.5094916
GTATAGG56850.014.407221
CTACACT97150.014.2550154
CTAGGAC63800.013.8636053
CTATACT111000.013.5214874
TAGACTG101700.013.4047015
TCTATAC110200.013.3552323
Produced by FastQC (version 0.11.5)