FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_7651.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_7651.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12839717
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA34650.065.9212956
CGGAGAT41150.059.923699
TCGGAGA41950.058.951058
CTCGGAG44700.049.6439257
GGCTCGG42000.047.6498225
GGGCTCG36550.043.6409764
CGTGGGC44400.024.8144531
GATCGGA12400.021.637076
TATACTG143000.015.2120125
CTATACT97750.015.2053454
TACACTG111950.014.5085955
GTATTAG94000.014.4968651
TACGCGG15050.014.445103145
CTACACT84350.013.8388484
GTATAGA97000.013.8243291
ATCGGAG18750.013.5368947
GTGTAGG63550.013.458931
GTCCTAC39350.013.4469021
CTTACAC81500.013.4321233
TCTATAC97500.013.3842253
Produced by FastQC (version 0.11.5)