FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_6852.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_6852.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17066207
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG195210.1143839401455754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA49450.078.307536
TCGGAGA53750.073.264868
CGGAGAT57050.071.0649
GGCTCGG54100.061.9225855
CTCGGAG64450.059.072677
GGGCTCG46350.057.4078184
CGTGGGC50650.029.0514771
GCCGACC38850.020.704948145
TGACGCG21350.020.026096145
GATCGGA12050.018.6553426
TACACTG144950.017.3086135
TATACTG198550.016.6897835
CGGCTCG13400.015.6909034
CCGTATA44850.015.672976145
CTACACT111700.015.6429484
CTTACAC107400.015.1877533
TAGACTG116850.014.8931625
TGGCTCG36600.014.4609384
GTGTAGG76950.014.2239411
TGGGCTC197450.014.1724663
Produced by FastQC (version 0.11.5)