FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_6852.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_6852.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21757847
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA68250.083.6184166
TCGGAGA75750.079.466538
CGGAGAT81100.076.016339
GGCTCGG73750.068.33355
CTCGGAG85200.066.4783557
GGGCTCG66400.061.3657764
CGTGGGC67000.039.5930061
GATCGGA19900.020.4063266
GCCGACC44150.019.03933145
TGGCTCG43400.017.8752464
GTGGGCT160850.017.3047492
CCGACGC110250.017.02337145
TGGGCTC256100.016.3900413
TACACTG196700.016.2571775
CGGCTCG19000.016.0270544
TATACTG258650.015.6434525
GACGACA55250.014.820761145
CTACACT139850.014.6717524
TCCGAGA33550.014.2672478
GTCCTAG98500.014.1278881
Produced by FastQC (version 0.11.5)